At right of center is a 300 liter computer controlled fermenter, (the vessel supplied by Dairy Crafts Inc. (DCI) of St. Cloud, MN), that has been instrumented with temperature, RPM, dissolved oxygen controlled by agitation or supplemental oxygen addition, antifoam control, pH control by acid/base or ammonia gas addition, automated nutrient addition, and with inlet and exhaust gas monitored by a process mass spectrometer. It is fully computer monitored and controlled (45, 68, 255).
At left is a 28.3 liter (New Brunswick Scientific Co., Inc.) fermenter that has been modified to operate with temperature, pH, dissolved oxygen, and antifoam control. This fermenter is also computer monitored. A variety of microorganisms have been grown in this bioreactor including: E-coli, yeast, Streptomyces, Bacillus, Pseudomonas, Lactobacillus, Azotobacter, Thrichoderma, Fusarium, Klebsiella, Gleotrichium, and Laccaria. Various fermentation modes have been employed including: programmed feeding, batch fermentation, continuous fermentation, fed-batch, plug flow, and as a chemostat (146, 195, 227, 229, 276).
Craig Bremmon
CEBTech Services
503 11 th Ave. SE, Suite 5
Aberdeen, SD 57401
bremm001@nvc.net Phone: 605 226-3595
Fax: 605 225-0467